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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
08/11/2011 |
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Data da última atualização: |
08/11/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CUNHA, B. C. A.; PASSOS, S. R.; RUMJANEK, N. G.; XAVIER, G. R. |
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Afiliação: |
BEATRIZ C. A. CUNHA, UFRRJ; SAMUEL R. PASSOS, UFRRJ; NORMA GOUVEA RUMJANEK, CNPAB; GUSTAVO RIBEIRO XAVIER, CNPAB. |
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Título: |
Análise via PCR-DGGE da microbiota associada à rizosfera de feijoeiro Olathe Pinto e Olathe Pinto 5.1 modificado geneticamente para resistência ao vírus do mosaico dourado. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 11., 2011, Seropédica. Mudanças climáticas, desastres naturais e prevenção de riscos: resumos... Seropédica: Embrapa Agrobiologia, 2011. |
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Páginas: |
36 p. |
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Idioma: |
Português |
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Palavras-Chave: |
16S rRNA; Alfaproteobactérias; DGGE; PCR. |
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Thesagro: |
Phaseolus Vulgaris. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/45794/1/ANALISE-VIA-PCR-DGGE.pdf
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Marc: |
LEADER 00820nam a2200205 a 4500
001 1905240
005 2011-11-08
008 2011 bl uuuu u00u1 u #d
100 1 $aCUNHA, B. C. A.
245 $aAnálise via PCR-DGGE da microbiota associada à rizosfera de feijoeiro Olathe Pinto e Olathe Pinto 5.1 modificado geneticamente para resistência ao vírus do mosaico dourado.$h[electronic resource]
260 $aSEMANA CIENTÍFICA JOHANNA DÖBEREINER, 11., 2011, Seropédica. Mudanças climáticas, desastres naturais e prevenção de riscos: resumos... Seropédica: Embrapa Agrobiologia$c2011
300 $a36 p.
650 $aPhaseolus Vulgaris
653 $a16S rRNA
653 $aAlfaproteobactérias
653 $aDGGE
653 $aPCR
700 1 $aPASSOS, S. R.
700 1 $aRUMJANEK, N. G.
700 1 $aXAVIER, G. R.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/04/2019 |
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Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SÁ, J. F. de; SAMPAIO, E. dos S.; MENDES, M. I. de S.; SANTOS, K. C. F. dos; SOUZA, A. da S.; LEDO, C. A. da S. |
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Afiliação: |
JUCIENY FERREIRA DE SÁ, UFRB; UFRB; MARIA INÊS DE SOUZA MENDES, UESC; KAREN CRISTINA FIALHO DOS SANTOS, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
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Título: |
Culture media for the multiplication of wild Manihot species meios de cultura para a multiplicação de espécies silvestres de Manihot. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, v.42, n.6, p.598-607, Nov./Dec. 2018 |
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ISSN: |
1981-1829 |
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Idioma: |
Inglês |
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Conteúdo: |
The cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species belonging to genus Manihot. MenosThe cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species... Mostrar Tudo |
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Thesagro: |
Cultura de Tecido; Mandioca; Micropropagação. |
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Thesaurus NAL: |
Micropropagation; Tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02332naa a2200253 a 4500
001 2107902
005 2019-12-06
008 2018 bl uuuu u00u1 u #d
022 $a1981-1829
100 1 $aSÁ, J. F. de
245 $aCulture media for the multiplication of wild Manihot species meios de cultura para a multiplicação de espécies silvestres de Manihot.$h[electronic resource]
260 $c2018
520 $aThe cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species belonging to genus Manihot.
650 $aMicropropagation
650 $aTissue culture
650 $aCultura de Tecido
650 $aMandioca
650 $aMicropropagação
700 1 $aSAMPAIO, E. dos S.
700 1 $aMENDES, M. I. de S.
700 1 $aSANTOS, K. C. F. dos
700 1 $aSOUZA, A. da S.
700 1 $aLEDO, C. A. da S.
773 $tCiência e Agrotecnologia$gv.42, n.6, p.598-607, Nov./Dec. 2018
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